Database   Three different vegetation systems will be studied: native tallgrass prairie, restored tallgrass prairie, and presently cultivated agricultural crops (Zea mays and Glycine max). Within each vegetation system, three soil sampling locations will be selected along a hillslope or topographic (drainage) gradient. Information that might be included in the databases: This will be a searchable database that includes information from all ascepts of this grant. The information will determine links between field and microbial community structure. Soil Characterization: Field Soil Morphology-horizon type, horizon depth intervals, structure, texture, color, consistence, roots, and redoximorphic features Laboratory Soil Characterization-pesticide/herbicide concentration and type, particle size distribution, pH, organic carbon, aggregate statibility, bulk density, total elemental analysis nutrients, C:N ratio Microbial community structure determined by: culturing techniques phospholipid fatty acid analysis (PFLA) analysis cloning/sequencing approach activity of microorganism through examination of RNA Biodegradation of agrochemicals: Types of microorganisms Environmental factors such as temperature, concentration of agrochemicals and trace nutrients, dissolved oxygen concentration, pH, and oxidation state of the minerals on the biodegradation of agrochemicals.